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Problem statement:

We want to upload images, including defocal pairs into the database

  • four types of data collection: tomogram, tiltseries (RCT/OTR), defocal pairs, and normal
  • current version does tiltseries (maybe tomogram series) and normal, but not defocal pairs

determine what the inputs are and what is required:

folder with mrc images -- only support mrc files

pixel size, specified in meters

tiltseries/defocal information (# per set) -- cannot have both

--images-per-tilt-series=3 --images-per-defocal-group=3

two session modes, create new session or append to existing session

* appending to a session is a dangerous things, so I am not going to support it.

* single mode simplifies things dramatically

input defocus and tilt angle:

* optionally allow a batch file for individual defocus and angle information

imagename <tab> defocus <tab> angle

* have override commandline flags

--angle-list=-45,0,45 --defocus-list=-2.0e-6,-4.0e-6,-6.0e-6

* not required to allow future uploading from the web

  1. four data collection modes: # tomographic tilt series: we cannot support uploading tomographic tilt series, pipeline requires Leginon information # RCT/OTR: requires a tiltseries connection # defocal pairs: require a AcquisitionImageTargetData connection, source:trunk/appion/appionlib/apDefocalPairs.py#L28 # normal mode: does not need any special treatment
  2. override session name???
  3. require a description

Create the program file

  • Find the feature request in redmine or file a new one
  • Copy appionScript.py.template
  • Make executable
  • Commit to subversion

Preliminary setup

  • Input options
  • Check conflicts

Setup program to do the work

  • Create session
  • Obtain Scope/Camera information
  • Uploading to the database

Test the program

Create web interface