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New User Features 30

Gatan K2 Summit support

Microcondenser mode setting in presets manager

Target Navigation:

  1. Option of using parent image move type to move to target during target adjustment.
    • This improves the targeting accuracy and minimize image-shift-induced beam-tilt in image-shifted final exposure if queuing is used in Exposure Targeting and Navigator iterative move is used in acquiring its parent image.
    • Activate it at /Leginon/Exposure/Settings/Advanced Settings: Use ancestor image mover in adjustment

Automatic Target Finding:

  1. Limit number of focusing performed on a series of images.
    • For example, focus once every 5 hl images that processed by Exposure Targeting node.
    • Iterator is reset at new parent so that hl image from a new square will always be focused.
    • Activate at /Leginon/Exposure Targeting/Settings/Advanced Settings/Hole Finder(or Raster Finder) Settings: Focus every nnn image. Default=1 meaning no skipping.
  2. Option to threshold very low intensity part of the image (grid bar or thick contaminant before template correlation in JAHCFinder used in MSI-T application
    • This has the effect of reducing false-positive in template-based hole finding.
    • Enter the threshold value at /Leginon/Exposure(or Hole) Targeting/Template Correlation Settings: Fill image values below n with mean before correlation.

Focusing

  1. Repeat same beam-tilt-based focusing step with the exactly same setting except not to drift checking will improve the focusing when the first adjustment is large
    • The first focusing step brings the defocus close to the range ideal for the algorithm for the repeat.
    • A bug fix makes this approach reliable and is recommended if defocus is really critical and speed of data acquisition does not out-weight it.

RCT Acquisition:

  1. Tilt Test Tool
    • Usage: For testing if the grid bars will block the intended targets when tilted. # Send the preset using for defining the RCT targets to scope with Presets Manager. # Each click of the Tilt Test Tool will take the goniometer to the next tilt defined in RCT settings and acquire an image.